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recombinant dna reagent atf4  (Addgene inc)


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    Addgene inc recombinant dna reagent atf4
    Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
    Recombinant Dna Reagent Atf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+dna+reagent+atf4/ATF4+1%3A+Mouse+ATF4+(CHOP11%2FcATF)-WT+(Plasmid+%2321845)/10__7554_slash_elife__63326-275-110-118
    Average 93 stars, based on 8 article reviews
    recombinant dna reagent atf4 - by Bioz Stars, 2026-08
    93/100 stars

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    1) Product Images from "The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals"

    Article Title: The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

    Journal: eLife

    doi: 10.7554/elife.63326

    Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
    Figure Legend Snippet: Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-

    Techniques Used: Knock-Out, Sequencing, Binding Assay, Generated

    Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page
    Figure Legend Snippet: Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page

    Techniques Used: Activation Assay, Western Blot, Transfection



    Similar Products

    recombinant dna reagent atf4  (Addgene inc)
    93
    Addgene inc recombinant dna reagent atf4
    Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
    Recombinant Dna Reagent Atf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+dna+reagent+atf4/ATF4+1%3A+Mouse+ATF4+(CHOP11%2FcATF)-WT+(Plasmid+%2321845)/10__7554_slash_elife__63326-275-110-118
    Average 93 stars, based on 1 article reviews
    recombinant dna reagent atf4 - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-

    Journal: eLife

    Article Title: The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

    doi: 10.7554/elife.63326

    Figure Lengend Snippet: Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-

    Article Snippet: DOI: https://doi.org/10.7554/eLife.63326 19 of 33 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct (mouse) siAtf4 GE Life Sciences/ Dharmacon L-042737-01-0020 Transfected construct (mouse) siRheb GE Life Sciences/ Dharmacon L-057044-00-0020 Transfected construct (mouse) siRhebL1 GE Life Sciences/ Dharmacon L-056074-01-0020 Transfected construct (mouse) siTsc2 GE Life Sciences/ Dharmacon L-047050-00-0020 Transfected construct (mouse) siC/ebpa GE Life Sciences/ Dharmacon L-040561-00-0005 Transfected construct (mouse) siC/ebpb GE Life Sciences/ Dharmacon L-043110-00-0005 Transfected construct (mouse) siC/ebpd GE Life Sciences/ Dharmacon L-060294-01-0005 Transfected construct (mouse) siC/ebpg GE Life Sciences/ Dharmacon L-065627-00-0005 Transfected construct (human) siATF4 GE Life Sciences/ Dharmacon L-005125-00-0020 Sequenced-based reagent qPCR primers IDT See table in Materials and methods Recombinant DNA reagent ATF4 (cDNA amplified from plasmid) Addgene RRID:Addgene_21845 Recombinant DNA reagent Pspax2 (plasmid) Addgene RRID:Addgene_12260 Recombinant DNA reagent Pmd2.G (plasmid) Addgene RRID:Addgene_12259 Recombinant DNA reagent pSpCas9n(BB) 2A-GFP (PX461) (plasmid) Addgene RRID:Addgene_48140 Recombinant DNA reagent pTRIPZ (plasmid) PMID:27088857 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent GFP (cDNA amplified from plasmid) Addgene RRID:Addgene_19319 Recombinant DNA reagent SLC7A11 (cDNA amplified from plasmid) PMID:29259101 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent pBabe hygro IRES-TSC2 PMID:15150095 David Kwiatkowski (Brigham and Women’s Hospital) Sequenced-based reagent CRISPR-Cas9n guides for KO of ATF4 IDT CACCGGAGG TGGAGGGGCTATGCT; AAACAGCATAGCCCC TCCACCTCC; CACCGACAATCTGCCTTC TCCAGG; AAACCC TGGAGAAGGCAGATTG TC Sequenced-based reagent Sequencing primers for Atf4-/- cell lines IDT TCGATGCTCTGTTTCGAA TG; CTTCTTCCCCC TTGCCTTAC Continued on next page Torrence et al. eLife 2021;10:e63326.

    Techniques: Knock-Out, Sequencing, Binding Assay, Generated

    Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page

    Journal: eLife

    Article Title: The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

    doi: 10.7554/elife.63326

    Figure Lengend Snippet: Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page

    Article Snippet: DOI: https://doi.org/10.7554/eLife.63326 19 of 33 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct (mouse) siAtf4 GE Life Sciences/ Dharmacon L-042737-01-0020 Transfected construct (mouse) siRheb GE Life Sciences/ Dharmacon L-057044-00-0020 Transfected construct (mouse) siRhebL1 GE Life Sciences/ Dharmacon L-056074-01-0020 Transfected construct (mouse) siTsc2 GE Life Sciences/ Dharmacon L-047050-00-0020 Transfected construct (mouse) siC/ebpa GE Life Sciences/ Dharmacon L-040561-00-0005 Transfected construct (mouse) siC/ebpb GE Life Sciences/ Dharmacon L-043110-00-0005 Transfected construct (mouse) siC/ebpd GE Life Sciences/ Dharmacon L-060294-01-0005 Transfected construct (mouse) siC/ebpg GE Life Sciences/ Dharmacon L-065627-00-0005 Transfected construct (human) siATF4 GE Life Sciences/ Dharmacon L-005125-00-0020 Sequenced-based reagent qPCR primers IDT See table in Materials and methods Recombinant DNA reagent ATF4 (cDNA amplified from plasmid) Addgene RRID:Addgene_21845 Recombinant DNA reagent Pspax2 (plasmid) Addgene RRID:Addgene_12260 Recombinant DNA reagent Pmd2.G (plasmid) Addgene RRID:Addgene_12259 Recombinant DNA reagent pSpCas9n(BB) 2A-GFP (PX461) (plasmid) Addgene RRID:Addgene_48140 Recombinant DNA reagent pTRIPZ (plasmid) PMID:27088857 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent GFP (cDNA amplified from plasmid) Addgene RRID:Addgene_19319 Recombinant DNA reagent SLC7A11 (cDNA amplified from plasmid) PMID:29259101 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent pBabe hygro IRES-TSC2 PMID:15150095 David Kwiatkowski (Brigham and Women’s Hospital) Sequenced-based reagent CRISPR-Cas9n guides for KO of ATF4 IDT CACCGGAGG TGGAGGGGCTATGCT; AAACAGCATAGCCCC TCCACCTCC; CACCGACAATCTGCCTTC TCCAGG; AAACCC TGGAGAAGGCAGATTG TC Sequenced-based reagent Sequencing primers for Atf4-/- cell lines IDT TCGATGCTCTGTTTCGAA TG; CTTCTTCCCCC TTGCCTTAC Continued on next page Torrence et al. eLife 2021;10:e63326.

    Techniques: Activation Assay, Western Blot, Transfection